Pengaruh Human Bone Marrow Mesenchymal Stem Cells Terhadap Penyembuhan Luka Bakar Pada Pembentukan Jaringan Granulasi Tikus DM

Dini Nurhasanah; Gusti Revilla; Deddy Saputra

doi:10.62027/vitamedica.v2i3.109

Authors

Dini Nurhasanah Universitas Andalas
Gusti Revilla Universitas Andalas
Deddy Saputra Universitas Andalas

DOI:

https://doi.org/10.62027/vitamedica.v2i3.109

Keywords:

Diabetes Mellitus, Human Bone Marrow Mesenchymal Stem Cells, Granulation Tissue, Burn

Abstract

Background: Burns with diabetes mellitus conditions can interfere with the wound healing process. Mesenchymal stem cells have been studied to heal burns, one of which originates from the bone marrow Objective: Determine the effect of human bone marrow mesenchymal stem cells on burn wound healing in the formation of granulation tissue DM rats. Methods: Research is experimental research with post-test only control group design. The research subjects were 30 rats divided into two groups, the control group was given NaCl and the treatment group was given hBM-MSCs. Mice were induced by alloxan to cause hyperglycemia, burns were made using a heated plate. Tissue collection was carried out after termination of the experimental animals on the 3rd, 7th, 14th days and then made histological preparations to assess the formation of granulation tissue. Data were analyzed using Two Way Anova Test. Results: Increase in the number of macrophages, fibroblasts and new blood vessels after hBM-MSCs were given. There were no increase on the 14th day neutrophils and 3rd day lymphocytes. Statistical analysis showed a significant increase in the number of lymphocytes, macrophages, fibroblasts and new blood vessels. Conclusion: The is study concludes that human bone marrow mesenchymal stem cells can increase the formation of granulation tissue by increasing macrophages, fibroblasts and new blood vessels in DM rats.

References

Abbas, O. L., Özatik, O., Gönen, Z. B., et al. (2018). Prevention of burn wound progression by mesenchymal stem cell transplantation: Deeper insights into underlying mechanisms. Annals of Plastic Surgery, 81(6), 715-724.

Afzali, L., Mirahmadi-Babaheydari, F., Shojaei-Ghahrizjani, F., Rahmati, S., Shahmoradi, B., & Banitalebi-Dehkordi, M. (2022). The effect of encapsulated umbilical cord-derived mesenchymal stem cells in PRP-Cryogel on regeneration of grade-II burn wounds. Regenerative Engineering and Translational Medicine, 8(1), 75-85.

Anggraeni, D., Airin, C. M., & Raharjo, S. (2018). The effectiveness of ethanol extract of binahong leaves on diabetic wound healing. Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Science, 11(4), 146-152.

Baltzis, D., Eleftheriadou, I., & Veves, A. (2014). Pathogenesis and treatment of impaired wound healing in diabetes mellitus: New insights. Advances in Therapy, 31(8), 817-836.

Barrientos, S., Stojadinovic, O., Golinko, M. S., Brem, H., & Tomic-Canic, M. (2008). Growth factors and cytokines in wound healing. Wound Repair and Regeneration, 16(5), 585-601.

Broughton, G., Janis, J. E., & Attinger, C. E. (2006). The basic science of wound healing. Plastic and Reconstructive Surgery, 117(7 SUPPL.), 12-34.

Chen, J. S., Wong, V. W., & Gurtner, G. C. (2012). Therapeutic potential of bone marrow-derived mesenchymal stem cells for cutaneous wound healing. Frontiers in Immunology, 3(JUL), 1-9.

Depkes RI. (2019). Pedoman Nasional Pelayanan Kedokteran Tatalaksana Luka Bakar. Jakarta.

Fitria, M., Saputra, D., & Revilla, G. (2014). Pengaruh papain getah pepaya terhadap pembentukan jaringan granulasi pada penyembuhan luka bakar tikus percobaan. Jurnal Kesehatan Andalas, 3(1), 73-76.

Fridoni, M., Kouhkheil, R., Abdollhifar, M. A., et al. (2019). Improvement in infected wound healing in type 1 diabetic rat by the synergistic effect of photobiomodulation therapy and conditioned medium. Journal of Cellular Biochemistry, 120(6), 9906-9916.

Gonzalez, A. C. D. O., Andrade, Z. D. A., Costa, T. F., & Medrado, A. R. A. P. (2016). Wound healing - A literature review. Anais Brasileiros de Dermatologia, 91(5), 614-620.

Gurtner, G. C. (2007). Wound healing normal and abnormal. In Thorne, C. H., Beasley, R. W., Aston, S. J., Bartlett, S. P., Gurtner, G. C., & Spear, S. L. (Eds.), Grabb and Smith’s Plastic Surgery (6th ed., pp. 15-22). Philadelphia: Lippincott Williams & Wilkins.

Hartono, B. (2016). Sel punca: Karakteristik, potensi dan aplikasinya. Jurnal Kedokteran Meditek, 22(60), 72-75.

Hasibuan, L. Y., & Soedjana, H. B. (2010). Luka. In Sjamsuhidajat, R., Karnadihardja, W., Prasetyono, T., & Rudiman, R. (Eds.), Buku Ajar Ilmu Bedah (3rd ed.). EGC.

Hosni Ahmed, H., Rashed, L. A., Mahfouz, S., et al. (2017). Can mesenchymal stem cells pretreated with platelet-rich plasma modulate tissue remodeling in a rat with burned skin? Biochemistry and Cell Biology, 95(5), 537-548.

Hu, M. S., Leavitt, T., Malhotra, S., et al. (2015). Stem cell-based therapeutics to improve wound healing. Plastic Surgery International, 2015, 1-7.

Husna, F., Suyatna, F. D., Arozal, W., & Purwaningsih, E. H. (2019). Model hewan coba pada penelitian diabetes. Pharmaceutical Sciences and Research, 6(3), 131-141.

Imbarak, N., Abdel-Aziz, H. I., Farghaly, L. M., & Hosny, S. (2021). Effect of mesenchymal stem cells versus aloe vera on healing of deep second-degree burn. Stem Cell Investigation, 8, 12-12.

Jiang, D., & Scharffetter-Kochanek, K. (2020). Mesenchymal stem cells adaptively respond to environmental cues thereby improving granulation tissue formation and wound healing. Frontiers in Cell and Developmental Biology, 8(July), 1-13.

Kareem, N. A., Aijaz, A., & Jeschke, M. G. (2021). Stem cell therapy for burns: Story so far. Biological Targets and Therapy, 15, 379-397.

Kong, P., Xie, X., Li, F., Liu, Y., & Lu, Y. (2013). Placenta mesenchymal stem cell accelerates wound healing by enhancing angiogenesis in diabetic Goto-Kakizaki (GK) rats. Biochemical and Biophysical Research Communications, 438(2), 410-419.

Krafts, K. P. (2010). Tissue repair: The hidden drama. Organogenesis, 6(4), 225-233.

Kuo, Y. R., Wang, C. T., Cheng, J. T., Kao, G. S., Chiang, Y. C., & Wang, C. J. (2016). Adipose-derived stem cells accelerate diabetic wound healing through the induction of autocrine and paracrine effects. Cell Transplantation, 25(1), 71-81.

Lee, D. E., Ayoub, N., & Agrawal, D. K. (2016). Mesenchymal stem cells and cutaneous wound healing: Novel methods to increase cell delivery and therapeutic efficacy. Stem Cell Research & Therapy, 7(1), 1-8.

Martins-Green, M., Petreaca, M., & Wang, L. (2013). Chemokines and their receptors are key players in the orchestra that regulates wound healing. Advances in Wound Care, 2(7), 327-347.

Muslim, S., Saputra, D., & Asri, A. (2021). Gambaran karakteristik pasien luka bakar listrik di rawat inap RSUP Dr. M. Djamil Padang tahun 2016-2019. Jurnal Ilmu Kesehatan Indonesia, 1(3), 412-418.

Oh, E. J., Lee, H. W., Kalimuthu, S., et al. (2018). In vivo migration of mesenchymal stem cells to burn injury sites and their therapeutic effects in a living mouse model. Journal of Controlled Release, 279, 79-88.

Prasetyono, T. O. H. (2009). General concept of wound healing. Medical Journal of Indonesia, 18(3), 208-216.

Primadina, N., Basori, A., & Perdanakusuma, D. S. (2019). Proses penyembuhan luka ditinjau dari aspek mekanisme seluler dan molekuler. Qanun Medika - Medical Journal of Faculty of Medicine Muhammadiyah Surabaya, 3(1), 31-43.

Revilla, G. (2018). Pengaruh bone marrow mesenchymal stem cells terhadap sekresi VEGF pada penyembuhan luka bakar tikus. Jurnal Kesehatan Andalas, 6(3), 702.

Roshangar, L., Rad, J. S., Kheirjou, R., Ranjkesh, M. R., & Khosroshasi, A. F. (2019). Skin burns: Review of molecular mechanisms and therapeutic approaches. Journal of Molecular Medicine, 31(12), 308-315.

Sierra-Sánchez, Á., Montero-Vilchez, T., Quiñones-Vico, M. I., Sanchez-Diaz, M., & Arias-Santiago, S. (2021). Current advanced therapies based on human mesenchymal stem cells for skin diseases. Frontiers in Cell and Developmental Biology, 9(March).

Stone, R., Natesan, S., Kowalczewski, C. J., et al. (2018). Advancements in regenerative strategies through the continuum of burn care. Frontiers in Pharmacology, 9(JUL).

Tanuwijaya, P. A., Ketut Berata, I., Agung, A., & Jayawardhita, G. (2019). Pemberian gel ekstrak daun binahong dalam proses angiogenesis penyembuhan luka insisi pada mencit hiperglikemia. Indonesian Medical Veterinary, 8(4), 2477-6637.

Velnar, T., Bailey, T., & Smrkolj, V. (2019). The wound healing process: An overview of the cellular and molecular mechanisms. Journal of International Medical Research, 37(5), 1528-1542.

Wan, J., Xia, L., Liang, W., Liu, Y., & Cai, Q. (2013). Transplantation of bone marrow-derived mesenchymal stem cells promotes delayed wound healing in diabetic rats. Journal of Diabetes Research, 2013.

Xu, J., Zgheib, C., Hodges, M. M., Caskey, R. C., Hu, J., & Liechty, K. W. (2017). Mesenchymal stem cells correct impaired diabetic wound healing by decreasing ECM proteolysis. Physiological Genomics, 49(10), 541-548. https://doi.org/10.1152/physiolgenomics.00090.2016

Yang, H. Y., Fierro, F., So, M., et al. (2020). Combination product of dermal matrix, human mesenchymal stem cells, and timolol promotes diabetic wound healing in mice. Stem Cells Translational Medicine, 9(11), 1353-1364.