Uji Efektivitas Analgetik Dekokta Herba Rumput Bambu (Lophatherum Gracile Brongn.) Terhadap Mencit Putih Jantan (Mus Musculus)

Siti Aisyah Tanjung; M.Gunawan; Safriana Safriana

doi:10.62027/vitamedica.v2i3.114

Authors

Siti Aisyah Tanjung Sekolah Tinggi Ilmu Kesehatan Indah Medan
M.Gunawan Sekolah Tinggi Ilmu Kesehatan Indah Medan
Safriana Safriana Sekolah Tinggi Ilmu Kesehatan Indah Medan

DOI:

https://doi.org/10.62027/vitamedica.v2i3.114

Keywords:

Analgesic, Dekokta, Bamboo grass herb, Metampiron

Abstract

Analgesics are compounds that in therapeutic doses relieve or suppress pain, without having general anesthetic action. Traditionally, the herb bamboo grass (Lophatherum gracile Brongn.) has been used and proven empirically to reduce pain, so it may have an analgesic effect. This can be caused by the content of secondary metabolite compounds, flavonoids, tannins, alkaloids, steroids/triterpenoids, saponins and glycosides, but this has not been scientifically proven. This research aims to determine the secondary metabolite compounds contained in fresh bamboo grass herbs, simplicia and dekokta and the effectiveness as an analgesic of the bamboo grass herb dekokta in mice.This research was carried out experimentally, including making simplicia and decoction, phytochemical screening and analgesic effectiveness testing. bamboo grass herb dekokta in male mice induced with 0.5% acetic acid intraperitoneally under the stomach, orally administered bamboo grass herb dekokta at a dose of 100 mg/kgBW, 200 mg/kgBW, 300 mg/kgBW, CMC 0.5% (blank), methampirone 65 mg/kgBB. Count the number of animal movements every 5 minutes for 1 hour. From the number of writhes obtained, the percentage of analgesic power and analgesic effectiveness were calculated. Next, the data obtained was analyzed statistically using SPSS ver.20 One Way ANOVA followed by the Tukey test. The results of the phytochemical screening of fresh bamboo grass, simplicia and dekokta contain alkaloids, flavonoids, tannins, saponins, steroids and glycosides. The greater the dose of bamboo grass herb dekokta given, the greater the percentage of reduction in writhing, so that the analgesic power and analgesic effectiveness are greater. . The results of the One Way ANOVA test and the Tukey test showed that the dose of 300 mg/kgBB had the best analgesic power at 50 minutes, not significantly different from administering 65 mg/kgBB of methampirone.

References

Afrianti, R., Yenti, R., & Meustika, D. (2014). Uji Aktivitas Analgetik Ekstrak Etanol Daun Pepaya (Carica Papaya L.) pada Mencit Putih Jantan yang di Induksi Asam Asetat 1%. Sains Farmasi dan Klinis, 1(1), 55–56.

Agoes, G. (2007). Teknologi Bahan Alam. ITB.

Anief, M. (1998). Ilmu Meracik Obat (Edisi VI). Gadjah Mada University Press.

Ansel, C. H. (2005). Pengantar Bentuk Sediaan Farmasi (Edisi IV). Universitas Indonesia Press.

Auliah, N., Latuconsina, A. A., & Thalib, M. (2019). Uji Efek Analgetik Ekstrak Etanol Daun Nangka (Artocarpus Heterophyllus Lam.) Terhadap Mencit (Mus Musculus) Yang Diinduksi Asam Asetat. STIKes Mega Rezky.

Bangun, A. (2012). Ensiklopedia Tanaman Obat Indonesia. Indonesia Publishing House.

Banziger, R. (1964). Animal Technique for Evaluating Narcotic and Non-narcotic Analgesics. In J. H. Nodine & P. E. Siregar (Eds.), Animal and Clinical Pharmacology Techniques in Drug Evaluation (pp. 52, 392–394). Year Book Medical Publishers.

Djauhariya, E., & Hernani. (2004). Gulma Berkhasiat Obat. Penebar Swadaya.

Domer, F. R., & Charles, C. (1971). Animal Experimental in Pharmacological Analysis (Edisi III).

Farnsworth, N. R. (1966). Biological and Phytochemical Screening of Plants. Journal of Pharmaceutical Science, 55(3), 253–264.

Ferdous, M., Rouf, R., Shilpi, J. A., & Uddin, S. J. (2008). Antinociceptive Activity of The Ethanolic Extract of Ficus Racemosa Lin (Moraceae). Oriental Pharmacy and Experimental Medicine, 93–96.

Harborne, J. B. (1987). Metode Fitokimia (Penuntun Cara Modern Menganalisis Tumbuhan) (Terbitan Kedua). ITB.

Jing, Z., Ying, W., Xiao-Qi, Z., Qing-Wen, Z., & Wen-Cai, Y. (2009). Chemical Constituents from the Leaves of Lophatherum Gracile. Chinese Journal of Natural Medicines, 7(6), 428.

Junaidi, I. (2013). Pedoman Praktis Obat Indonesia. Bhuana Ilmu Populer.

Kamiensky, M., & Keogh, J. (2006). Farmakologi Demystified. Rapha Publishing.

Mutschler, E. (1991). Dinamika Obat Buku Ajar Farmakologi dan Toksikologi (Edisi V, Terj. Widianto, M. B., & Ranti, A. S. S.). ITB.

Parmadi, A., & Nadiarti, A. (2015). Uji Daya Analgetik Ekstrak Etanol Daun Seledri (Apium Graveolens L.) Pada Mencit Galur Swiss dengan Metode Rangsang Kimia. Indonesian Journal on Medical Science, 100.

Robinson, T. (1995). Kandungan Organik Tumbuhan Tinggi (Edisi ke-4, Terj. Kosasih Padmawinata). ITB.

Savitri, A. (2016). Tanaman Ajaib Basi Penyakit dengan Toga (Tanaman Obat Keluarga). Bibit Publisher.

Sentat, T., & Pangestu, S. (2016). Uji Efek Analgesik Ekstrak Etanol Daun Kersen (Muntingia Calabura L.) Pada Mencit Putih Jantan (Mus Musculus L.) Dengan Induksi Nyeri Asam Asetat. Jurnal Ilmiah Manuntung, 2(2), 147–153.

Setiadi. (2017). Dasar-dasar Farmakologi untuk Keperawatan. Indomedica Pustaka.

Syamsuni, H. A. (2006). Ilmu Resep. EGC.

Tjay, H. T., & Rahardja, K. (2007). Obat-obat Penting Khasiat Penggunaan dan Efek-efek Sampingnya (Edisi Keenam). PT. Elex Media Komputindo.

Traditional Medicine Journal. (n.d.). Vol 21(2). Akademi Farmasi.

Turner, R. A. (1965). Screening Methods in Pharmacology. Academic Press.

Wahyuni, T. A. Y., & Nuratmi, B. (2003). Uji Perbandingan Efek Analgesik Infus Temu Putih (Curcuma Zedoaria Rosc.) dan Temu Mangga (Curcuma Manga Val. Et Zipp) Pada Mencit. Jurnal Bahan Alam Indonesia, 2(3), 81–84.